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Lipase Production from Bacillus subtilis using various Agricultural waste( Vol-3,Issue-5,May 2017 ) |
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Author(s): Naaz Abbas, Javeria Javed, Zainab Abbas, Shahnaz Choudry, Sakhawat Ali |
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Keywords: |
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Lipase, Bacillus subtilis, Surface Fermentation, agricultural wastes, optimization. |
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Abstract: |
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Lipases was produced by Bacillus subtilis PCSIR NL-38 strain and rape seed oil cake as substrate. Surface fermentation of minimal media in 250ml conical flask under static conditions gave 12.81 U/ml of lipases at 40°C for 48 hours. Lipase activity was monitored titrimatrically. Optimization of physicochemical parameters indicated that PCSIR NL-38 showed maximum lipase production at pH 7 with NH4NO3 as inorganic nitrogen source, glucose as carbon source, FeSO4.7H2O as salt, with 7% inoculum size and 96 hours of incubation. |
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