Lipase Production from Bacillus subtilis using various Agricultural waste

( Vol-3,Issue-5,May - May 2017 )

Author(s): Naaz Abbas, Javeria Javed, Zainab Abbas, Shahnaz Choudry, Sakhawat Ali

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Page No: 405-409
DOI: 10.24001/ijaems.3.5.1

Keywords:

Lipase, Bacillus subtilis, Surface Fermentation, agricultural wastes, optimization.

Abstract:

Lipases was produced by Bacillus subtilis PCSIR NL-38 strain and rape seed oil cake as substrate. Surface fermentation of minimal media in 250ml conical flask under static conditions gave 12.81 U/ml of lipases at 40°C for 48 hours. Lipase activity was monitored titrimatrically. Optimization of physicochemical parameters indicated that PCSIR NL-38 showed maximum lipase production at pH 7 with NH4NO3 as inorganic nitrogen source, glucose as carbon source, FeSO4.7H2O as salt, with 7% inoculum size and 96 hours of incubation.

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